mgh:cyto-2-3

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Cytology Rotation 2-3 Immunochemical stains in cytopathology

Immunochemistry is used frequently in the work up of cytology samples, but given that most immunochemical staining protocols were developed for histologic sections and controls are usually histologic tissue sections, one needs to be aware of the factors that will impact immunocytochemical stains.

For a given cytologic preparation (cell block, direct smears, liquid based cytology) the issues with immunochemical stain will vary.

Cell block

Direct smear

Liquid based cytology