Week 1
- Content Expert: David C. Wilbur, MD
- Cytotechnologist: Peter Brown
- Objective: To describe the common morphologic features in glandular cell lesions found in pap smears.
Related PDFs of Lectures
Normal endocervicals – MG07-41719
- Column cells with basally located nuclei
- Basophilic vacuolated cytoplasm
- Picket fence arrangement when seen on side, honeycomb when seen from above
- Nuclei round, oval, slightly larger than em nuclei, similar to sq. met.
Normal endometrial cells – MG07-37747
- Nuclei small, round, dark, indistinct nucleoli
- Cytoplasm is scant, may have vacuoles
- Deep stromal cells, dark, degenerate with scant cytoplasm
- Superficial stromal cells look like histocytes
- 3-D ball like clusters
Reactive endocervicals – C02-X21800
- Slightly to moderately enlarged nuclear size
- Prominent nucleoli
- Can see multinucleation
- Chromatin normal
- Nuclear contours regular
- In groups cells maintain polarity
Endocervical atypia 10D –C92-1561 and MG09-21470
- Changes are seen in endometrial cells or endocervical cells, em’s shed at mid cycle
- Single cells or small clusters of reaction endocervicals
- Normal or slightly irregular ems with vacuoles
- Actinomyces
Tubal metaplasia – G11-37675
- Single ec’s cells, strips of ec’s with pseudostratification or in clusters with either terminal bars or cilia
- Nuclei can be round or oval
- Slightly coarse chromatin (without the presence of cilia or terminal bars can be mistaken for AIS)
AIS endocervical – MG06-00971 and C01-D39098
- Columnar cells in crowded groups, strips on rosettes
- Strips may have pseudostratification, groups with irregular polarity
- Peripheral nuclear palisading (feathering)
- Irregular dark chromatin, nuclear enlargement
Endocervial Adenoca – MG09-31908
- Ec’s with enlarged hyperchromatic nuclei in strips
- Rosettes, pseudostratification, feathering
- Loss of polarity
- Difficult to differentiate from AIS
Endocervical Adenoca – C98-17153
- Strips, rosettes, feathering
- Disorderly arrangement
- High N/C ratio
- Coarse chromatin
- Architectural features similar to AIS but with greater loss of polarity
- Prominent nucleoli
- Mitosis
- Diasisis
Endometrial CA Grade III – C02-R1948
- Ball like and papillary groups of irregular glandular cells
- Nuclear contour irregularities
- Prominent nucleoli
- Watery tumor diathesis
Metastatic ovarian adenocarcinoma – C02-N42569
- Papillary architecture
- Nuclear enlargement
- Crowding
- Loss of polarity
- Psammoma bodies may be present
