Difference between revisions of "mgh:cyto-1-7"
From MGH Learn Pathology
| (4 intermediate revisions by the same user not shown) | |||
| Line 1: | Line 1: | ||
__NOCACHE__{{DISPLAYTITLE:1-7 Respiratory Cytology: W.S. Black-Schaffer MD, Mary Rego CT}}{{:TOC}} | __NOCACHE__{{DISPLAYTITLE:1-7 Respiratory Cytology: W.S. Black-Schaffer MD, Mary Rego CT}}{{:TOC}} | ||
== Lectures == | == Lectures == | ||
| − | * [ | + | * [https://web.microsoftstream.com/video/af91a85a-4326-4941-bc21-7b104a895178 Lecture: Respiratory cytology, part 1 by Dr. WS Black-Schaffer] |
| − | * [ | + | * [https://web.microsoftstream.com/video/aaed0c76-e865-4199-a092-9464eb494912 Lecture: Respiratory cytology, part 2. by Dr. W.S. Black-Schaffer] |
* Indications for cytology examination | * Indications for cytology examination | ||
| Line 10: | Line 10: | ||
<br> | <br> | ||
== Basic cytomorphology == | == Basic cytomorphology == | ||
| − | + | {{collapsed| | |
| − | + | '''Cytology Respiratory Specimens –MN05-J8970'''| | |
| − | '''Cytology Respiratory Specimens –MN05-J8970''' | ||
* Sputum | * Sputum | ||
* Bronchial washing and brushings | * Bronchial washing and brushings | ||
* Bronchoalveolar lavage (BAL) | * Bronchoalveolar lavage (BAL) | ||
* Fine needle aspirations (FNA) | * Fine needle aspirations (FNA) | ||
| − | + | |}} | |
| − | + | {{collapsed| | |
| − | '''Normal Respiratory Cytology –MN05-X13303''' | + | '''Normal Respiratory Cytology –MN05-X13303'''| |
* Squamous cells (oral contamination) | * Squamous cells (oral contamination) | ||
* Glandular cells | * Glandular cells | ||
| Line 26: | Line 25: | ||
* Pneumocytes | * Pneumocytes | ||
* Alveolar macrophages | * Alveolar macrophages | ||
| − | + | |}} | |
| − | + | {{collapsed| | |
| − | '''Alveolar macrophages –MN06-M2366''' | + | '''Alveolar macrophages –MN06-M2366'''| |
* Oval to bean shaped nuclei | * Oval to bean shaped nuclei | ||
* Mononucleated or multinucleated | * Mononucleated or multinucleated | ||
| Line 36: | Line 35: | ||
* siderophages | * siderophages | ||
* lipophages(associated with aspiration pneumonia) | * lipophages(associated with aspiration pneumonia) | ||
| − | + | |}} | |
| − | + | {{collapsed| | |
| − | '''Granulomatous Inflammation –MN05-F5872''' | + | '''Granulomatous Inflammation –MN05-F5872'''| |
* Seen in pt’s with TB, Sarcoid and Rheumatoid Arthritis. | * Seen in pt’s with TB, Sarcoid and Rheumatoid Arthritis. | ||
* Can see giant cells | * Can see giant cells | ||
* Nodular collections of epithelioid histiocytes | * Nodular collections of epithelioid histiocytes | ||
* Cells with elongated nuclei with pale chromatin and tiny nucleoli | * Cells with elongated nuclei with pale chromatin and tiny nucleoli | ||
| − | + | |}} | |
| − | + | {{collapsed| | |
| − | '''Chondroid Hamartoma–C03-C14116''' | + | '''Chondroid Hamartoma–C03-C14116'''| |
* Cartilage | * Cartilage | ||
* mature - homogenous, waxy | * mature - homogenous, waxy | ||
| Line 52: | Line 51: | ||
* small bland bronchial cells | * small bland bronchial cells | ||
* Respiratory Infection –MN08-F9275 and N12-9869 | * Respiratory Infection –MN08-F9275 and N12-9869 | ||
| − | + | |}} | |
| − | + | {{collapsed| | |
| − | '''Candida and Actinomyces from oral contamination''' | + | '''Candida and Actinomyces from oral contamination'''| |
* Aspergillus | * Aspergillus | ||
* 45º angle branching of true septate | * 45º angle branching of true septate | ||
| Line 64: | Line 63: | ||
* broad, irregular ribbon-like hyphae | * broad, irregular ribbon-like hyphae | ||
* folding of hyphae | * folding of hyphae | ||
| − | + | |}} | |
| − | + | {{collapsed| | |
| − | '''Squamous Cell Carcinoma –C92-8306 and MN06-324''' | + | '''Squamous Cell Carcinoma –C92-8306 and MN06-324'''| |
* Keratinizing (Well-Diff)/ Nonkeratinizing(Mod to poorly Diff) | * Keratinizing (Well-Diff)/ Nonkeratinizing(Mod to poorly Diff) | ||
* Bizarre cell shapes | * Bizarre cell shapes | ||
| Line 73: | Line 72: | ||
* Coarsely granular and/or opaque nucleus | * Coarsely granular and/or opaque nucleus | ||
* Nucleoli possible | * Nucleoli possible | ||
| − | + | |}} | |
| − | + | {{collapsed| | |
| − | '''Bronchogenic adenocarcinoma –C00-A43961''' | + | '''Bronchogenic adenocarcinoma –C00-A43961'''| |
* 3-D balls, single cells, syncytia | * 3-D balls, single cells, syncytia | ||
* Lacy, fine, vacuolated cytoplasm | * Lacy, fine, vacuolated cytoplasm | ||
| Line 81: | Line 80: | ||
* Finely granular irregular chromatin | * Finely granular irregular chromatin | ||
* Nucleoli | * Nucleoli | ||
| − | + | |}} | |
| − | + | {{collapsed| | |
| − | '''Bronchioloalveolar Carcinoma –C01-W33204''' | + | '''Bronchioloalveolar Carcinoma –C01-W33204'''| |
* 3-D balls, papillary growth | * 3-D balls, papillary growth | ||
* Finely granular to clear cytoplasm | * Finely granular to clear cytoplasm | ||
| Line 90: | Line 89: | ||
* Intranuclear cytoplasmic invaginations | * Intranuclear cytoplasmic invaginations | ||
* Psammoma bodies may be seen | * Psammoma bodies may be seen | ||
| − | + | |}} | |
| − | + | {{collapsed| | |
| − | '''Small Cell Carcinoma –C03-T32870 and MN04-G5451''' | + | '''Small Cell Carcinoma –C03-T32870 and MN04-G5451'''| |
* Single and syncytial | * Single and syncytial | ||
* Nuclear molding | * Nuclear molding | ||
| Line 101: | Line 100: | ||
* Crush artifact ( nuclear DNA streaming) | * Crush artifact ( nuclear DNA streaming) | ||
* One to four times the size of a lymphocyte | * One to four times the size of a lymphocyte | ||
| − | + | |}} | |
| − | + | {{collapsed| | |
| − | '''Carcinoid –C02-M34743''' | + | '''Carcinoid –C02-M34743'''| |
* Uniform cuboidal cells | * Uniform cuboidal cells | ||
* Scant to moderate cytoplasm | * Scant to moderate cytoplasm | ||
| Line 110: | Line 109: | ||
* Small nucleoli possible | * Small nucleoli possible | ||
* Small blood vessels in background | * Small blood vessels in background | ||
| − | + | |}} | |
| − | + | {{collapsed| | |
| − | '''Metastatic malignancy –MN07-T9423, MN07-T9423 and C97-B18744''' | + | '''Metastatic malignancy –MN07-T9423, MN07-T9423 and C97-B18744'''| |
* Compare the metastatic with the primary lesion | * Compare the metastatic with the primary lesion | ||
* Usually no tumor diathesis | * Usually no tumor diathesis | ||
* Presence of multiple nodules and a history of morphologically compatible cancer strongly favor metastasis | * Presence of multiple nodules and a history of morphologically compatible cancer strongly favor metastasis | ||
| + | |}} | ||
{{:mgh:cytology-footer}} | {{:mgh:cytology-footer}} | ||
Latest revision as of 13:17, July 6, 2020
Contents
Lectures
- Lecture: Respiratory cytology, part 1 by Dr. WS Black-Schaffer
- Lecture: Respiratory cytology, part 2. by Dr. W.S. Black-Schaffer
- Indications for cytology examination
- Procuring the specimen
- Test platforms/specimen processing and triage
- Report
Basic cytomorphology
Cytology Respiratory Specimens –MN05-J8970
- Sputum
- Bronchial washing and brushings
- Bronchoalveolar lavage (BAL)
- Fine needle aspirations (FNA)
Normal Respiratory Cytology –MN05-X13303
- Squamous cells (oral contamination)
- Glandular cells
- ciliated columnar
- mucous goblet cells
- Pneumocytes
- Alveolar macrophages
Alveolar macrophages –MN06-M2366
- Oval to bean shaped nuclei
- Mononucleated or multinucleated
- Foamy cytoplasm
- Phagocytic cells
- carbon histiocytes
- siderophages
- lipophages(associated with aspiration pneumonia)
Granulomatous Inflammation –MN05-F5872
- Seen in pt’s with TB, Sarcoid and Rheumatoid Arthritis.
- Can see giant cells
- Nodular collections of epithelioid histiocytes
- Cells with elongated nuclei with pale chromatin and tiny nucleoli
Chondroid Hamartoma–C03-C14116
- Cartilage
- mature - homogenous, waxy
- immature - fibrillar texture
- Epithelium
- small bland bronchial cells
- Respiratory Infection –MN08-F9275 and N12-9869
Candida and Actinomyces from oral contamination
- Aspergillus
- 45º angle branching of true septate
- Pneumocystis
- foamy alveolar casts
- GMS- stain cell wall of cyst black
- cysts are 4 to 8 um
- Zygomycetes-(Mucor)
- broad, irregular ribbon-like hyphae
- folding of hyphae
Squamous Cell Carcinoma –C92-8306 and MN06-324
- Keratinizing (Well-Diff)/ Nonkeratinizing(Mod to poorly Diff)
- Bizarre cell shapes
- Cytoplasm is dense or hard with defined cell borders
- Heavy keratinization(seen in Keratinizing SCC)
- Coarsely granular and/or opaque nucleus
- Nucleoli possible
Bronchogenic adenocarcinoma –C00-A43961
- 3-D balls, single cells, syncytia
- Lacy, fine, vacuolated cytoplasm
- Cuboidal cells, eccentric nuclei
- Finely granular irregular chromatin
- Nucleoli
Bronchioloalveolar Carcinoma –C01-W33204
- 3-D balls, papillary growth
- Finely granular to clear cytoplasm
- Eccentric nuclei
- Pale to moderately hyperchromatic chromatin
- Intranuclear cytoplasmic invaginations
- Psammoma bodies may be seen
Small Cell Carcinoma –C03-T32870 and MN04-G5451
- Single and syncytial
- Nuclear molding
- Scant cytoplasm
- Oval to angulated nuclei
- Nucleoli not prominent
- Dense chromasia (salt and pepper chromatin)
- Crush artifact ( nuclear DNA streaming)
- One to four times the size of a lymphocyte
Carcinoid –C02-M34743
- Uniform cuboidal cells
- Scant to moderate cytoplasm
- Small round to oval nuclei
- Stippled granular chromatin
- Small nucleoli possible
- Small blood vessels in background
Metastatic malignancy –MN07-T9423, MN07-T9423 and C97-B18744
- Compare the metastatic with the primary lesion
- Usually no tumor diathesis
- Presence of multiple nodules and a history of morphologically compatible cancer strongly favor metastasis
